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To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
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Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Crystal-Templated Colloidal Clusters Exhibit Directional DNA Interactions.

James T McGinley1, Yifan Wang1, Ian C Jenkins1

  • 1Department of Chemical and Biomolecular Engineering, University of Pennsylvania , 220 South 33rd Street, Philadelphia, Pennsylvania 19104, United States.

ACS Nano
|October 7, 2015
PubMed
Summary
This summary is machine-generated.

Researchers created DNA-functionalized colloidal clusters with five distinct symmetries using a crystal template. These clusters exhibit directional binding, enabling their use as building blocks for advanced self-assembly applications.

Keywords:
DNAcolloidal clustersdirected assemblydirectional interactionsligation

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Area of Science:

  • Colloid science
  • Materials science
  • Nanotechnology

Background:

  • Spherical colloids with grafted DNA facilitate directed self-assembly of crystal and gel structures.
  • Anisotropic building blocks can increase the diversity of colloidal assemblies.
  • DNA-functionalized spheres offer programmable interactions for self-assembly.

Purpose of the Study:

  • To create diverse colloidal clusters with specific symmetries using DNA-functionalized spheres.
  • To explore the self-assembly capabilities of these clusters as building blocks.
  • To demonstrate the directional binding properties of the DNA-coated clusters.

Main Methods:

  • Sedimentation of binary mixtures of host and impurity spheres within a colloidal crystal template.
  • Hybridization and enzymatic ligation of DNA strands between sphere species.
  • Centrifugation for separation of desired colloidal clusters.

Main Results:

  • Formation of five distinct colloidal cluster symmetries: tetrahedra, octahedra, cuboctahedra, triangular orthobicupola, and icosahedra.
  • Clusters exhibit symmetry determined by the nearest neighbors in the template crystal.
  • Demonstrated directional binding of clusters due to uniformly grafted DNA strands.

Conclusions:

  • A scalable method for fabricating colloidal clusters with controlled symmetries and directional DNA interactions was developed.
  • These clusters serve as versatile building blocks for subsequent directed self-assembly.
  • The reprogrammable and directional DNA interactions mimic natural binding processes.