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Updated: Apr 1, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Efficient Gene Editing in Primary Human T Cells.

Yvonne Y Chen¹

¹Department of Chemical and Biomolecular Engineering, University of California-Los Angeles, Los Angeles, CA 90095, USA.

Trends in Immunology

|October 7, 2015

Summary

This summary is machine-generated.

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Efficient genome editing in primary human T cells is now possible using CRISPR and TALEN technologies. This breakthrough advances T-cell engineering for treating cancer, infections, and autoimmune diseases.

Area of Science:

Immunology
Genetics
Biotechnology

Background:

T-cell engineering holds significant therapeutic promise for various diseases, including cancer, viral infections, and autoimmune disorders.
Precise genetic modification of primary human T cells is crucial for realizing this potential but has been technically challenging.
Recent advancements aim to overcome these limitations in T-cell manipulation.

Purpose of the Study:

To describe and evaluate efficient genome editing techniques in primary human T cells.
To highlight the application of CRISPR and TALEN technologies for T-cell modification.
To facilitate the development of novel T-cell-based therapies.

Main Methods:

Utilized CRISPR-Cas9 genome editing system for targeted DNA modification in primary human T cells.

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Related Experiment Videos

Last Updated: Apr 1, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Published on: April 13, 2017

Employed Transcription Activator-Like Effector Nucleases (TALENs) for site-specific genetic alterations in primary human T cells.

Assessed the efficiency and specificity of genome editing in primary human T cells.

Main Results:

Demonstrated high-efficiency genome editing in primary human T cells using both CRISPR and TALEN approaches.
Confirmed site-specific genetic modifications, indicating precision in T-cell engineering.
Established robust protocols for genetic manipulation of primary human T cells.

Conclusions:

CRISPR and TALEN technologies offer efficient and precise methods for genome editing in primary human T cells.
These advancements are expected to accelerate the development and application of T-cell therapies.
Overcoming technical hurdles in T-cell manipulation opens new avenues for treating complex diseases.