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SecA protein is directly involved in protein secretion in Escherichia coli
H Kawasaki1, S Matsuyama, S Sasaki
1Institute of Applied Microbiology, University of Tokyo, Japan.
FEBS Letters
|January 2, 1989
Abstract:
A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.