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Temporal small RNA transcriptome profiling unraveled partitioned miRNA expression in developing maize endosperms
Mingming Xin1, Guanghui Yang1, Yingyin Yao1
1State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University Beijing, China.
Frontiers in Plant Science
|October 7, 2015
Summary
MicroRNAs (miRNAs) are crucial for maize endosperm development, showing dynamic expression patterns between reciprocal crosses. A specific miRNA, Zma-miR408-5p, exhibits biased expression, potentially impacting gene regulation.
Area of Science:
- Plant Molecular Biology
- Genomics
- Developmental Biology
Background:
- The endosperm is vital for seed development in angiosperms, providing nourishment to the embryo.
- Small interfering RNAs, including microRNAs (miRNAs), are key regulators of gene expression.
- Previous studies generated small RNA sequencing data for developing maize endosperm.
Purpose of the Study:
- To investigate the roles of miRNAs in regulating gene expression during maize endosperm development.
- To analyze the expression patterns of conserved and novel miRNAs in maize endosperm.
- To identify potential functional variations in miRNAs between maize inbred lines.
Main Methods:
- High-throughput small RNA sequencing of maize kernels and endosperms at various developmental stages (0-15 days after pollination).
- Bioinformatic analysis to identify and quantify miRNA expression.
- Quantitative RT-PCR, SNP analysis, and CAPS experiments to validate expression patterns and variations.
Main Results:
- 57 conserved and 18 novel miRNAs were highly expressed in maize endosperm.
- miRNAs displayed dynamic and stage-specific expression patterns, differing between reciprocal crosses (B73 and Mo17).
- A single stem-loop structure can generate distinct tandem miRNAs, potentially conserved in monocots.
- Zma-miR408-5p showed a B73-biased expression pattern, linked to SNP variation.
Conclusions:
- MicroRNAs play a significant role in regulating maize endosperm development.
- Distinct miRNA expression patterns exist between reciprocal maize crosses, influencing endosperm development.
- MiRNA sequence variations, like in Zma-miR408-5p, can lead to differential gene regulation.
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