Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Metabolism01:40

Overview of Metabolism

41.1K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
41.1K
What is Metabolism?00:52

What is Metabolism?

136.3K
Overview
136.3K
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

1.2K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.2K
Regulation of Metabolism01:19

Regulation of Metabolism

12.4K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
12.4K
Introduction to Metabolism01:30

Introduction to Metabolism

3.6K
Metabolism encompasses all biochemical reactions in a living organism, facilitating both the breakdown and synthesis of biomolecules. These metabolic processes are categorized into catabolic and anabolic pathways, which operate in a coordinated manner to ensure energy balance and cellular function.Catabolic Pathways and Energy ReleaseCatabolic pathways involve the breakdown of complex macromolecules such as carbohydrates, lipids, and proteins into smaller structures like monosaccharides, fatty...
3.6K
Metabolic Rate01:25

Metabolic Rate

1.4K
The human body is a powerhouse of energy, with every cell performing numerous functions that require energy. This energy production and consumption is measured by the metabolic rate, which quantifies the total heat generated by all the body's chemical reactions and mechanical work. This measurement helps to determine the rate of kilocalorie (kcal) consumption needed to fuel all ongoing activities.
The Basal Metabolic Rate (BMR) measures the energy expended at rest.
Several factors influence...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering an Extremely Hybrid PKS for Adipic Acid Production.

ACS synthetic biology·2026
Same author

Assembly and Reactions of Artificial Metalloenzymes in <i>Streptomyces albus</i>.

Journal of the American Chemical Society·2026
Same author

Polyketide synthase-based controlled synthesis of polycyclopropanated fuel molecules.

Nature communications·2026
Same author

Expanding the scope of redox-balance growth coupling techniques with a carbon co-feeding strategy.

Metabolic engineering·2026
Same author

Design and commissioning of a new synchrotron beamline dedicated to X-ray footprinting mass spectrometry.

Journal of synchrotron radiation·2026
Same author

Expanding the scope of redox-balance growth coupling techniques with a carbon cofeeding strategy.

bioRxiv : the preprint server for biology·2026
Same journal

Discerning dangerous gain of function: most gain of function (GoF) research does not involve infectious microbes.

Frontiers in bioengineering and biotechnology·2026
Same journal

Microtopography screening to modulate the mitogenic effects of aqueous humor on human tenon fibroblasts.

Frontiers in bioengineering and biotechnology·2026
Same journal

Next-generation strategies for anterior cruciate ligament repair: constructing biointelligent ligament grafts integrating biomimetic design, immune modulation, and sensory feedback.

Frontiers in bioengineering and biotechnology·2026
Same journal

Collagen nanofiber reinforced alginate hydrogel tube microbioreactors for cell culture.

Frontiers in bioengineering and biotechnology·2026
Same journal

Calcium ions released from alginate hydrogel promote wound healing by enhancing fibroblast activity.

Frontiers in bioengineering and biotechnology·2026
Same journal

Application and validation of AI-assisted 3D-Printed gastroduodenal anatomical variation models in specialized nursing training.

Frontiers in bioengineering and biotechnology·2026
See all related articles

Related Experiment Video

Updated: Apr 1, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K

Analytics for Metabolic Engineering.

Christopher J Petzold1, Leanne Jade G Chan1, Melissa Nhan1

  • 1Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA , USA.

Frontiers in Bioengineering and Biotechnology
|October 7, 2015
PubMed
Summary
This summary is machine-generated.

Advancing metabolic engineering requires well-defined biological parts and analytical tools. Combining screening and omics analysis improves strain design and construction for robust, viable systems.

Keywords:
RNA-seqhigh-throughput screeningmetabolic engineeringmetabolomicsmicrofluidicsproteomics

More Related Videos

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

3.8K
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
07:06

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Published on: August 7, 2018

10.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

4.4K
Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

3.8K
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
07:06

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Published on: August 7, 2018

10.1K

Area of Science:

  • Metabolic Engineering
  • Synthetic Biology
  • Biotechnology

Background:

  • Metabolic engineering advances are hindered by challenges in developing robust, economically viable systems beyond proof-of-concept.
  • Current research relies heavily on analytical tools to assay molecules, transcripts, proteins, and metabolites.
  • A need exists for well-defined biological parts with predictable performance across diverse contexts.

Purpose of the Study:

  • To highlight the critical role of analytical tools and well-defined biological parts in advancing metabolic engineering.
  • To emphasize the importance of quantitative data for informed decision-making in strain design and construction.
  • To accelerate the transition from trial-and-error research to predictable and scalable metabolic engineering.

Main Methods:

  • Utilizing high-throughput screening technologies to analyze thousands of strain variants.
  • Employing deep omics analysis for a systems-level understanding of cellular functions.
  • Integrating data from screening and omics to quantify dynamic processes between biological parts and host chassis.

Main Results:

  • Screening technologies provide specific data on numerous strain variants.
  • Deep omics analysis offers a comprehensive, systems-level view of engineered cells.
  • Combined analyses yield quantitative insights into part-host interactions.

Conclusions:

  • Access to well-defined biological parts with predictable metrics is crucial for progress.
  • Analytical tools, including screening and omics, are essential for quantitative data generation.
  • Data-driven decision-making accelerates the design and construction of improved metabolic engineering systems.