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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Updated: Apr 1, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Genome Editing in Rats Using TALE Nucleases.

Laurent Tesson^1,2,3, Séverine Remy^4,5,6, Séverine Ménoret^4,5,6

¹Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.

Methods in Molecular Biology (Clifton, N.J.)

|October 8, 2015

Summary

This summary is machine-generated.

Transcription activator-like (TALE) nucleases enable efficient generation of knockout and knock-in rats for disease modeling. This cost-effective method accelerates the creation of valuable new rat models for research.

Keywords:

Gene editing Genetic engineering Genomics Homology-directed repair Hprt Knock-in Knockout Nonhomologous end joining Rat model Rosa26 TALE nucleases TALEN Targeted integration

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Published on: December 11, 2020

Area of Science:

* Genetics and Genomics
* Animal Models in Biomedical Research
* Molecular Biology

Background:

* Rats are crucial animal models for understanding gene function and human diseases.
* Gene-specific nucleases are vital tools for creating novel rat models.
* Transcription activator-like (TALE) nucleases offer precise gene editing capabilities.

Purpose of the Study:

* To detail a protocol for generating knockout and knock-in rats using TALE nucleases.
* To highlight the efficiency and cost-effectiveness of this technology for creating disease models.

Main Methods:

* Microinjection of TALE nucleases into fertilized rat eggs.
* Utilizing TALE nucleases for gene knockout creation.
* Employing homology-directed recombination (HDR) for generating knock-in rat models.

Main Results:

* Successful generation of gene-specific knockout rats.
* Demonstrated feasibility of generating knock-in rats via HDR.
* TALE nuclease technology proves efficient for rat model creation.

Conclusions:

* TALE nucleases provide an efficient method for creating knockout and knock-in rats.
* This technology is cost- and time-effective for developing new rat models.
* Facilitates advancements in studying gene function and modeling human diseases in rats.