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Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

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The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
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Electron Carriers01:24

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Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
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Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

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Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
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EDTA: Auxiliary Complexing Reagents01:26

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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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The Citric Acid Cycle02:36

The Citric Acid Cycle

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The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
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Redox Reactions01:27

Redox Reactions

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Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
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Related Experiment Video

Updated: Apr 1, 2026

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
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NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

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NADH as Donor.

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    Summary
    This summary is machine-generated.

    Escherichia coli has two NADH dehydrogenases (NDH-I and NDH-II) involved in energy transduction. This review details their properties and mechanisms, particularly NDH-I

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Escherichia coli utilizes two primary respiratory NADH dehydrogenases, NDH-I and NDH-II, with ongoing debate regarding their specific roles in energy transduction.
    • NDH-I (Complex I) functions in both aerobic and anaerobic respiration, whereas NDH-II is specifically repressed during anaerobic growth.

    Purpose of the Study:

    • To review the properties and mechanisms of the two NADH dehydrogenases in E. coli.
    • To elucidate the energy-converting mechanism of NDH-I using high-resolution structural data.
    • To discuss the physiological implications of NDH-II regulation and its impact on metabolic pathways.

    Main Methods:

    • Review of existing literature on NADH dehydrogenases in E. coli.
    • Analysis of high-resolution structural data for the soluble portion of NDH-I.
    • Examination of mutant studies, including Salmonella enterica serovar Typhimurium complex I mutants.

    Main Results:

    • E. coli possesses two distinct respiratory NADH dehydrogenases: NDH-I and NDH-II.
    • NDH-I is crucial for both aerobic and anaerobic respiration, while NDH-II is active primarily under aerobic conditions.
    • Insufficient NADH recycling due to NDH-II repression can inhibit key metabolic enzymes and affect cellular processes like proteolysis.

    Conclusions:

    • The distinct roles and regulation of NDH-I and NDH-II are critical for E. coli energy metabolism.
    • Understanding these enzymes provides insights into bacterial respiration and metabolic regulation.
    • NDH-II, a single-subunit enzyme, requires specific conditions for activity and purification, highlighting its unique characteristics.