Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

19.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
19.0K
Replication in Eukaryotes02:31

Replication in Eukaryotes

208.0K
Overview
208.0K
Replication in Eukaryotes02:31

Replication in Eukaryotes

54.1K
54.1K
Replication in Eukaryotes01:29

Replication in Eukaryotes

11.7K
11.7K
The DNA Replication Fork01:02

The DNA Replication Fork

43.2K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
43.2K
The DNA Replication Fork01:02

The DNA Replication Fork

20.3K
20.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recollections of a Helmstetter Disciple.

Life (Basel, Switzerland)·2023
Same author

Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During the <i>Escherichia coli</i> Cell Cycle by Transcriptional Modulators That Interact With Origin DNA.

Frontiers in microbiology·2021
Same author

Editorial: DNA Replication Origins in Microbial Genomes, Volume 2.

Frontiers in microbiology·2019
Same author

Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.

Frontiers in microbiology·2019
Same author

Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy.

Antibiotics (Basel, Switzerland)·2019
Same author

Low Affinity DnaA-ATP Recognition Sites in <i>E. coli oriC</i> Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication.

Frontiers in microbiology·2018
Same journal

A history of <i>EcoSal Plus</i>.

EcoSal Plus·2025
Same journal

Transcriptional reprogramming by bacteriophage T4: turning the host transcriptional machinery to the dark side.

EcoSal Plus·2025
Same journal

Bacteriophage T4 genome packaging: mechanism and application.

EcoSal Plus·2025
Same journal

The bacteriophage T4 homologous recombination system: mechanism, applications, conservation, and environmental significance.

EcoSal Plus·2025
Same journal

The bacteriophage T4 replisome: a model system for understanding DNA replication mechanisms.

EcoSal Plus·2025
Same journal

Biology of host-dependent restriction-modification in prokaryotes.

EcoSal Plus·2025
See all related articles

Related Experiment Video

Updated: Apr 1, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

Initiation of DNA Replication.

Alan C Leonard, Julia E Grimwade

    Ecosal Plus
    |October 8, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Complex regulatory circuits control DNA replication initiation. This chapter details the bacterial initiator protein DnaA, its interactions at the origin of replication (oriC), and mechanisms preventing premature DNA replication.

    More Related Videos

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
    07:18

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

    Published on: October 27, 2011

    40.8K
    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
    08:53

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

    Published on: May 2, 2025

    1.1K

    Related Experiment Videos

    Last Updated: Apr 1, 2026

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
    07:27

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

    Published on: April 29, 2010

    14.0K
    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
    07:18

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

    Published on: October 27, 2011

    40.8K
    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
    08:53

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

    Published on: May 2, 2025

    1.1K

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • DNA replication initiation is a complex, tightly regulated process.
    • The bacterial initiator protein DnaA plays a crucial role in this process.
    • Understanding the regulation of DNA replication is vital for cell viability.

    Purpose of the Study:

    • To review recent advancements in understanding the regulation of DNA replication initiation.
    • To explore the protein-DNA interactions at the origin of replication (oriC).
    • To discuss the structural and biochemical properties of the bacterial initiator protein DnaA.

    Main Methods:

    • Analysis of temperature-sensitive, conditional-lethal mutants of E. coli.
    • Identification and characterization of replication-defective genes (dnaA and dnaC).
    • Biochemical and structural studies of DnaA protein and oriC interactions.

    Main Results:

    • Newly identified nucleotide sequence features within E. coli oriC.
    • Characterization of structural and biochemical attributes of the bacterial initiator protein DnaA.
    • Insights into mechanisms preventing improperly timed DNA replication.

    Conclusions:

    • The DnaA protein is the essential bacterial initiator.
    • Regulation of DnaA accessibility to oriC and its assembly at oriC are critical.
    • Further research is needed to fully elucidate DnaA's role in oriC unwinding and replication initiation.