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Updated: Apr 1, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
Enhancing our understanding of enhancers in T-helper cells
Jacob J Paiano1,2, John L Johnson1,2, Golnaz Vahedi1,2
1Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Abstract:
A long-standing question in immunology has been to understand how transcription factors (TF) determine cell-type-specific transcription programs. Traditionally, investigating TFs in immune cells has been limited to measuring the effect of a single TF on a limited number of gene targets. The advent of next generation sequencing methods makes it possible to measure the effects of multiple transcription factors on a genome-wide scale. This holistic approach gives us a better understanding of the influence that multiple TFs have on cell-specific programs. In this issue of European Journal of Immunology, Fang et al. [Eur. J. Immunol. 2015. 45: 3150-3157] show that genomic regions cooccupied with two or more TFs show increased epigenetic histone marks of active enhancers, which correspond to increased transcriptional activity.
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