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Updated: Apr 1, 2026
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
Induction Function of miR-126 in Survival and Proliferation in Neural Stem Cells
Qijun Zhang1, Sheng Zeng1, Chengyuan Quan1
1Department of Emergency, Pingyang People's Hospital, Wenzhou, Zhejiang, China (mainland).
Background:
The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs).
Material And Methods:
Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-counting kit-8 (CCK8) assay, colony formation assays. Flow cytometry was performed to evaluate the effect of miR-126 on cell survival.
Results:
CCK8 assay and colony formation assay showed that overexpression of miR-126 promoted cell proliferation and increased colony numbers in HB1.F3 and HB1.A4 cells. The flow cytometry confirmed the results that miR-126 inhibited cell apoptosis.
Conclusions:
MiR-126 promoted the proliferation and survival of NSCs.
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