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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Updated: Apr 1, 2026

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

Mesenchymal Stem Cells Derived from Human Bone Marrow.

Oliver F W Gardner^1,2, Mauro Alini¹, Martin J Stoddart³

¹Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|October 9, 2015

Summary

This summary is machine-generated.

Mesenchymal stem cells (MSCs) from human bone marrow are isolated and expanded. Their multipotency for tissue engineering, especially bone and cartilage repair, is characterized.

Keywords:

Adipogenesis Chondrogenesis Mesenchymal stem cell Osteogenesis

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Last Updated: Apr 1, 2026

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

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Published on: February 9, 2015

Isolating Mesangiogenic Progenitor Cells MPCs from Human Bone Marrow

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Published on: July 15, 2016

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Published on: August 31, 2022

Area of Science:

Biomedical Engineering
Cell Biology
Regenerative Medicine

Background:

Mesenchymal stem cells (MSCs) are multipotent stromal cells found in various tissues.
Their differentiation potential makes them valuable for tissue engineering applications.
Bone and cartilage repair are key areas of interest for MSC-based therapies.

Purpose of the Study:

To describe the isolation of MSCs from human bone marrow.
To detail the methods for expanding these cells in vitro.
To characterize the multipotency of isolated and expanded MSCs.

Main Methods:

Isolation of MSCs from human bone marrow aspirates.
In vitro expansion of MSC cultures.
Assessment of MSC differentiation into mesenchymal lineages (e.g., osteoblasts, chondrocytes).

Main Results:

Successful isolation and expansion of viable MSCs from human bone marrow.
Demonstration of MSCs' capacity to differentiate into multiple mesenchymal lineages.
Characterization confirms the multipotent nature of the cells.

Conclusions:

Human bone marrow is a viable source for MSC isolation and expansion.
Expanded MSCs retain their multipotency, supporting their use in tissue engineering.
These findings facilitate further research into MSC-based regenerative therapies.