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Flow cytometry for the study of phagocyte functions.

R Bjerknes1, C F Bassøe, H Sjursen

  • 1Medical Department B, University of Bergen, Haukeland Hospital, Norway.

Reviews of Infectious Diseases
|January 1, 1989
PubMed
Summary

Flow cytometry enables detailed analysis of phagocyte functions, crucial for host defense against infections. This technique allows researchers to study various cellular processes and interactions with microorganisms, aiding in understanding immune cell defects.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Phagocytes are critical for host defense, with defects leading to increased infection susceptibility.
  • Flow cytometry provides rapid, reproducible single-cell analysis of multiple cell functions simultaneously.

Purpose of the Study:

  • To present flow-cytometric assays for comprehensive study of phagocyte functions and interactions with microorganisms.
  • To highlight the utility of flow cytometry in both experimental and clinical immunology.

Main Methods:

  • Utilizing fluorochromes for multi-parameter analysis of phagocyte subpopulations.
  • Employing specialized computer systems for data storage and detailed analysis.
  • Developing specific assays to assess phagocyte surface receptors, membrane potential, phagocytosis, and intracellular processes.

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Main Results:

  • Established assays enable detailed examination of phagocyte surface receptors, membrane potential, and phagocytosis (attachment vs. internalization).
  • Methods allow assessment of phagosomal pH, degranulation, enzymatic activity, intracellular calcium, oxidative metabolism, and microbial killing/degradation.
  • The influence of serum opsonins on phagocyte-microorganism interactions can be effectively studied.

Conclusions:

  • Flow cytometry is a versatile tool for dissecting complex phagocyte functions and host-pathogen interactions.
  • These techniques are valuable for both basic research into immune cell defects and clinical applications in diagnosing infectious disease susceptibility.