Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

10.7K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
10.7K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.7K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The clinical spectrum of HbSC sickle cell disease-not a benign condition.

British journal of haematology·2024
Same author

Zinc regulates reactive oxygen species generation in platelets.

Platelets·2020
Same author

The chaperone protein HSP47: a platelet collagen binding protein that contributes to thrombosis and hemostasis.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Platelet collagen receptor Glycoprotein VI-dimer recognizes fibrinogen and fibrin through their D-domains, contributing to platelet adhesion and activation during thrombus formation.

Journal of thrombosis and haemostasis : JTH·2017
Same author

Implementation of internet-delivered cognitive behavior therapy within community mental health clinics: a process evaluation using the consolidated framework for implementation research.

BMC psychiatry·2017
Same author

Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.

Journal of thrombosis and haemostasis : JTH·2017

Related Experiment Video

Updated: Apr 1, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

18.8K

Modified platelet deposition on matrix metalloproteinase 13 digested collagen I.

J-M Howes1, N Pugh2, V Knäuper3

  • 1Department of Biochemistry, University of Cambridge, Cambridge, UK.

Journal of Thrombosis and Haemostasis : JTH
|October 9, 2015
PubMed
Summary

Collagen degradation by MMP-13 impacts platelet adhesion and thrombus formation. Partial degradation enhances thrombus deposition, while complete degradation abolishes platelet reactivity.

Keywords:
GPVI collagen receptorcollagencollagenasematrix metalloproteinase 13platelets

More Related Videos

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

1.3K
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
12:25

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Published on: June 2, 2017

10.5K

Related Experiment Videos

Last Updated: Apr 1, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 27, 2009

18.8K
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

1.3K
2D and 3D Matrices to Study Linear Invadosome Formation and Activity
12:25

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Published on: June 2, 2017

10.5K

Area of Science:

  • Biochemistry
  • Hematology
  • Cardiovascular Biology

Background:

  • Atherothrombosis, a key factor in acute coronary syndromes, involves plaque rupture and subsequent thrombus formation.
  • Monocytes within unstable plaques release matrix metalloproteinases (MMPs), such as MMP-13, which degrade collagen.
  • Degraded collagen fragments exposed after plaque rupture can influence platelet activation and thrombus development.

Purpose of the Study:

  • To investigate the impact of collagen degradation on platelet adhesion and thrombus formation.
  • To understand how matrix metalloproteinase-13 (MMP-13) mediated collagen breakdown affects platelet aggregation.

Main Methods:

  • Progressive degradation of collagen I using varying concentrations of MMP-13.
  • Electrophoretic visualization of collagen degradation.
  • Assessment of platelet adhesion and thrombus formation on collagen fragments using whole flowing blood under static and flow conditions.

Main Results:

  • Fibrous collagen supported high platelet adhesion, largely unaffected by MMP-13 treatment.
  • Monomeric collagen adhesion was dependent on the α2β1 integrin.
  • Partial degradation of monomeric collagen increased thrombus deposition under flow, but complete degradation abolished this effect.

Conclusions:

  • Partial digestion of collagen monomers exposes new binding sites that influence platelet interactions.
  • Complete collagen degradation leads to a loss of net platelet reactivity.
  • Understanding these collagen-platelet dynamics is crucial for managing atherothrombotic events.