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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Parameterization of hyperpolarized (13)C-bicarbonate-dissolution dynamic nuclear polarization.

David Johannes Scholz1, Angela M Otto1, Josef Hintermair1

  • 1Institute of Medical Engineering, Technische Universität München, Munich, Germany.

Magma (New York, N.Y.)
|October 10, 2015
PubMed
Summary

Hyperpolarized carbon-13 (13C) bicarbonate MRI allows preclinical pH detection. Optimized preparation and procedures improved signal-to-noise ratio (SNR) and T1 values for in vitro and in vivo imaging.

Keywords:
Cell spheroidsHyperpolarized 13CMCF-7NMR spectroscopy

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Area of Science:

  • Biomedical Imaging
  • Metabolic Imaging
  • Magnetic Resonance Imaging

Background:

  • Carbon-13 (13C) metabolic MRI with hyperpolarized 13C-bicarbonate is a preclinical tool for pH detection.
  • Improving the signal-to-noise ratio (SNR) is crucial for enhancing the utility of this technique.
  • Understanding factors influencing SNR, such as pH, buffer capacity, temperature, and field strength, is essential.

Purpose of the Study:

  • To optimize the preparation of hyperpolarized 13C-bicarbonate for enhanced MRI performance.
  • To investigate the influence of various experimental parameters on signal quality and stability.
  • To demonstrate the feasibility of in vivo pH mapping using this optimized approach.

Main Methods:

  • Investigated bicarbonate preparation techniques to maximize solubility and polarization.
  • Performed spectroscopy at varying field strengths (1, 3, 14 T) using different solutions (pure dissolution, culture medium, cell spheroids).
  • Conducted in vivo imaging in healthy rats using spectral-spatial spiral acquisition for spatial and temporal analysis.

Main Results:

  • Developed an optimized preparation yielding 6 mol/L solubility and 19-21% polarization.
  • Characterized T1 relaxation time and SNR dependencies on field strength, buffer capacity, and pH.
  • Successfully demonstrated in vivo pH mapping in rats.

Conclusions:

  • Optimized bicarbonate preparation and experimental protocols significantly improved T1 and SNR.
  • The enhanced methodology enables robust in vitro and in vivo applications for metabolic MRI.
  • This advancement facilitates more sensitive preclinical pH detection using hyperpolarized 13C-bicarbonate MRI.