Related Experiment Video
Updated: Apr 1, 2026
In Vitro Thrombosis Test for Ventricular Assist Devices
Published on: March 21, 2025
In Vitro Evaluation of Intra-Aneurysmal, Flow-Diverter-Induced Thrombus Formation: A Feasibility Study
K Gester1, I Lüchtefeld2, M Büsen2
1From the Department of Cardiovascular Engineering (K.G., I.L., M.B., S.J.S., T.L., U.S.), Institute of Applied Medical Engineering, Helmholtz Institute-RWTH Aachen University, Aachen, Germany kathrin.gester@rwth-aachen.de.
Background And Purpose:
Intracranial aneurysm treatment by flow diverters aims at triggering intra-aneurysmal thrombosis. By combining in vitro blood experiments with particle imaging velocimetry measurements, we investigated the time-resolved thrombus formation triggered by flow diverters.
Materials And Methods:
Two test setups were built, 1 for particle imaging velocimetry and 1 for blood experiments, both generating the same pulsatile flow and including a silicone aneurysm model. Tests without flow diverters and with 2 different flow-diverter sizes (diameter: 4.5 and 4.0 mm) were performed. In the blood experiments, the intra-aneurysmal flow was monitored by using Doppler sonography. The experiments were stopped at 3 different changes of the spatial extent of the signal.
Results:
No thrombus was detected in the aneurysm model without the flow diverter. Otherwise, thrombi were observed in all aneurysm models with flow diverters. The thrombi grew from the proximal side of the aneurysm neck with fibrin threads connected to the flow diverter and extending across the aneurysm. The thrombus resulting from the 4.0-mm flow diverter grew along the aneurysm wall as a solid and organized thrombus, which correlates with the slower velocities near the wall detected by particle imaging velocimetry. The thrombus that evolved by using the 4.5-mm flow diverter showed no identifiable growing direction. The entire thrombus presumably resulted from stagnation of blood and correlates with the central vortex detected by particle imaging velocimetry.
Conclusions:
We showed the feasibility of in vitro investigation of time-resolved thrombus formation in the presence of flow diverters.
