Genome sequence of Streptomyces pluripotens MUSC 135(T) exhibiting antibacterial and antioxidant activity

Area of Science:

• Microbiology
• Genomics
• Natural Products Chemistry

Background:

• Intertidal soil harbors diverse microorganisms with potential for producing bioactive compounds.
• The emergence of antibiotic-resistant pathogens necessitates the discovery of novel antimicrobial agents.
• Streptomyces species are known producers of a wide array of secondary metabolites, including antibiotics and antioxidants.

Purpose of the Study:

• To isolate and characterize a novel Streptomyces strain from intertidal soil.
• To investigate the antimicrobial and antioxidant properties of the isolated strain.
• To perform a genomic analysis to identify genes involved in the production of bioactive compounds.

Main Methods:

• Isolation and cultivation of Streptomyces pluripotens MUSC 135(T) from intertidal soil.
• Determination of antibacterial spectrum against key pathogens, including methicillin-resistant Staphylococcus aureus (MRSA).
• Fermentation studies to assess antioxidant production.
• Whole-genome sequencing and bioinformatics analysis to identify secondary metabolite gene clusters.

Main Results:

• Streptomyces pluripotens MUSC 135(T) demonstrated broad-spectrum antibacterial activity against MRSA, Salmonella typhi, and Aeromonas hydrophila.
• Fermentation broths of MUSC 135(T) exhibited significant antioxidant activity.
• The high-quality draft genome (7,480,269 bp, 70.00% G+C content) revealed 72 gene clusters associated with secondary metabolite production.

Conclusions:

• Streptomyces pluripotens MUSC 135(T) is a promising source of novel bacteriocins and antioxidants with potential therapeutic applications.
• The genomic data provides a foundation for understanding the biosynthetic pathways of its bioactive compounds.
• Further research into these secondary metabolites could lead to the development of new antimicrobial and antioxidant agents.