Tyrosinase-based biosensor for determination of bisphenol A in a flow-batch system

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Bisphenol A (BPA) is an endocrine disruptor found in various environmental and food samples.
• Accurate determination of BPA is crucial for environmental monitoring and public health.

Purpose of the Study:

• To develop and characterize a tyrosinase-based amperometric biosensor for sensitive and reliable BPA determination.
• To investigate the analytical performance of the biosensor in a flow-batch sequential injection system.

Main Methods:

• Immobilization of tyrosinase enzyme in a sol-gel TiO2 matrix modified with multi-walled carbon nanotubes (MWCNTs), poly(diallyldimethylammonium chloride) (PDDA), and Nafion.
• Morphological characterization using scanning electron microscopy (SEM).
• Electrochemical analysis and assessment of analytical parameters including linear range, sensitivity, limit of detection, stability, repeatability, and reproducibility.

Main Results:

• The developed biosensor exhibited a linear range for BPA detection from 0.28 to 45.05 µM.
• High sensitivity of 3263 µA mM(-1) cm(-2) and a low limit of detection of 0.066 µM were achieved.
• The biosensor demonstrated good long-term stability, repeatability, and reproducibility.

Conclusions:

• The fabricated tyrosinase/TiO2/MWCNTs/PDDA/Nafion biosensor is effective for BPA determination.
• The sequential injection system coupled with the biosensor provides a robust platform for analyzing BPA in natural samples.