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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Colorimetric As (V) detection based on S-layer functionalized gold nanoparticles.

Mathias Lakatos¹, Sabine Matys², Johannes Raff³

¹Dresden University of Technology, Institute of Materials Science, Max Bergmann Center of Biomaterials, Budapester Strasse 27, 01069 Dresden, Germany.

|October 11, 2015

Summary

This summary is machine-generated.

Simple colorimetric and UV/VIS methods detect anionic arsenic (V) using S-layer-functionalized gold nanoparticles. These nanoparticles aggregate, causing a color change, enabling sensitive arsenic detection in water.

Keywords:

Arsenic Colorimetric Detection Nanoparticle S-layer

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Area of Science:

Nanotechnology
Analytical Chemistry
Environmental Science

Background:

Arsenic contamination in water poses significant health risks.
Developing rapid and sensitive detection methods for arsenic is crucial for public health and environmental monitoring.

Purpose of the Study:

To develop simple, rapid, and sensitive colorimetric and UV/VIS spectroscopic methods for detecting anionic arsenic (V) complexes.
To utilize S-layer-functionalized gold nanoparticles for arsenic detection.

Main Methods:

Functionalization of gold nanoparticles (20-50 nm) with S-layer protein oligomers from Lysinibacillus sphaericus JG-A12.
Exploiting nanoparticle aggregation in the presence of arsenic (V) complexes, leading to a color change from red to blue.
Utilizing UV/VIS spectroscopy to analyze the shift in the particle plasmon resonance signal for quantitative detection.

Main Results:

The methods provide a visual color change (burgundy-red to blue) upon arsenic (V) complex aggregation.
UV/VIS spectroscopy allows for detailed signal analysis and sensitive detection.
Using 50 nm gold nanoparticles, concentrations of anionic arsenic (V) as low as 24 ppb were detectable.

Conclusions:

S-layer-functionalized gold nanoparticles offer a promising platform for the rapid and sensitive detection of arsenic (V) in aqueous media.
The developed colorimetric and spectroscopic methods are simple, efficient, and suitable for on-site arsenic monitoring.
Further optimization with larger nanoparticles can enhance detection sensitivity for environmental applications.