A highly sensitive fluorescence turn-on platform with silver nanoparticles aptasening for human platelet-derived...

In this paper, we demonstrated a simple and highly sensitive fluorescence platform for protein detection. Silver nanoparticles (AgNPs) worked as carriers and quenchers for FAM labeled aptamers (FAM-apt). Biotin labeled aptamers (Bio-apt), FAM-apt functionalized AgNPs (Ag-FAM-apt), and a target protein, human platelet-derived growth factor-BB (PDGF-BB) could form a sandwich-type complex. Once the etching solvents were added, AgNPs were dissolved and the fluorescence resonance energy transfer (FRET) between AgNPs and FAM was broken. FAM-apt were no longer quenched and released into the solution in the 96-well microplates, so the fluorescence signal would turn from "off" state to "on" state. This method had possessed several advantages: Firstly, increased specificity which was contributed by the sandwich binding of aptamers; Secondly, quenching ability of AgNPs which was utilized to make signal turn-on; Thirdly, high throughout in which 96 samples could be detected simultaneously. The results showed a linear relationship between fluorescence intensity and PDGF-BB concentration (10 ng mL(-1)-100 ng mL(-1)), and the detection limit was 7 ng mL(-1). This simple and sensitive method would have a promising future for development and application.