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Complement-mediated inhibition of function in complement-resistant Escherichia coli.

J R Dankert1

  • 1Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610.

Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1989
PubMed
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Complement (C) inhibits E. coli by forming the membrane attack complex (MAC), damaging inner membrane function. Resistance mechanisms may involve reversing MAC

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Complement-mediated inhibition of E. coli involves membrane attack complex (MAC) assembly.
  • Inner membrane function inhibition is crucial for complement-induced cell death.
  • Outer membrane damage alone is insufficient for cell killing without serum lysozyme.

Purpose of the Study:

  • To investigate the role of inner membrane function inhibition in complement-sensitive E. coli.
  • To explore mechanisms of cellular resistance to complement attack.
  • To determine if reversal of MAC inhibitory effects contributes to complement resistance.

Main Methods:

  • Measuring oxygen consumption in E. coli under complement attack.
  • Analyzing MAC assembly and its impact on cellular respiration.

Related Experiment Videos

  • Observing transient inner membrane function inhibition in complement-resistant strains.

    • Main Results:

    • Complement-sensitive E. coli showed inhibited oxygen consumption due to MAC assembly, indicating inner membrane damage.
    • Damage to the outer membrane alone did not cause cell death.
    • Complement-resistant cells exhibited transient inhibition of inner membrane function.

    Conclusions:

    • Complement-mediated inner membrane dysfunction is critical for killing E. coli.
    • Cellular resistance to complement may involve reversing MAC-induced inhibition.
    • Transient inner membrane inhibition in resistant cells suggests a role for reversal mechanisms.