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Updated: Apr 1, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Eomesodermin Expression in CD4+ T Cells Restricts Peripheral Foxp3 Induction.

Ekaterina Lupar¹, Maria Brack², Laure Garnier³

¹Max-Planck-Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany; Center for Chronic Immunodeficiency, University Medical Center Freiburg and University of Freiburg, D-79106 Freiburg, Germany; Faculty of Biology, University of Freiburg, D-79104 Freiburg, Germany;

Journal of Immunology (Baltimore, Md. : 1950)

|October 11, 2015

Summary

This summary is machine-generated.

The transcription factor Eomes limits the development of regulatory T cells (Tregs) in CD4(+) T cells. Eomes deficiency enhances Treg accumulation and improves protection against autoimmune diseases like experimental autoimmune encephalitis.

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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

CD4(+) T cells differentiate into distinct effector subsets, regulated by specific transcription factors.
While T-bet's role in Th1 cells is known, eomesodermin (Eomes) function in CD4(+) T cells remains unclear.

Purpose of the Study:

To investigate the expression and function of Eomes in mouse CD4(+) T lymphocytes.
To determine Eomes's role in regulating T cell differentiation and immune responses.

Main Methods:

Analysis of Eomes expression in activated CD4(+) T cells in vivo.
Genetic deletion of Eomes in CD4(+) T cells.
In vitro differentiation of naive CD4(+) T cells.
Assessment of regulatory T cell (Foxp3(+) cells) induction and function.
Experimental autoimmune encephalitis (EAE) model to evaluate T cell-mediated autoimmunity.

Main Results:

Eomes is expressed in activated CD4(+) Th1 T cells in vivo and accumulates under specific conditions (aging, lymphopenia, oral antigen administration).
Eomes deficiency in CD4(+) T cells did not affect IFN-γ production or Th2/Th17 responses but promoted Foxp3(+) cell accumulation.
Enforced Eomes expression reduced Foxp3 transcription in vitro.
Eomes-deficient CD4(+) T cells showed enhanced protection against EAE, associated with increased Foxp3(+) cell frequency.

Conclusions:

Eomes acts as a novel regulator in CD4(+) T cells, primarily by limiting Foxp3 induction.
Eomes expression may influence susceptibility to autoimmune diseases, potentially through its effect on regulatory T cell populations.