Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

9.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.2K
MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.7K
MicroRNAs01:22

MicroRNAs

12.0K
12.0K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

3.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
3.2K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.3K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of MicroRNA-4697-3p by Parkinson's disease-associated SNP rs329648 and its impact on SNCA112 mRNA.

Molecular biology reports·2024
Same author

MicroRNA-593-5p contributes to cell death following exposure to 1-methyl-4-phenylpyridinium by targeting PTEN-induced putative kinase 1.

The Journal of biological chemistry·2023
Same author

MicroRNA-7 Protects Against Neurodegeneration Induced by α-Synuclein Preformed Fibrils in the Mouse Brain.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2021
Same author

MicroRNA-7 promotes motor function recovery following spinal cord injury in mice.

Biochemical and biophysical research communications·2021
Same author

Transglutaminase 2 Depletion Attenuates α-Synuclein Mediated Toxicity in Mice.

Neuroscience·2020
Same author

Translation of the intrinsically disordered protein α-synuclein is inhibited by a small molecule targeting its structured mRNA.

Proceedings of the National Academy of Sciences of the United States of America·2020

Related Experiment Video

Updated: Apr 1, 2026

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

24.7K

MicroRNA-7 activates Nrf2 pathway by targeting Keap1 expression.

Savan Kabaria1, Doo Chul Choi1, Amrita Datta Chaudhuri1

  • 1Center for Neurodegenerative and Neuroimmunologic Diseases, Department of Neurology, Rutgers-Robert Wood Johnson Medical School, Piscataway, NJ., 08854, USA.

Free Radical Biology & Medicine
|October 11, 2015
PubMed
Summary

MicroRNA-7 (miR-7) represses Keap1, increasing the activity of the antioxidant transcription factor Nrf2 (Nuclear factor E2-related factor 2). This mechanism provides cellular protection against oxidative stress and neurotoxicity.

Keywords:
Keap1MPP+MicroRNA-7Nrf2Oxidative stressParkinson's disease

Related Experiment Videos

Last Updated: Apr 1, 2026

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

24.7K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Nuclear factor E2-related factor 2 (Nrf2) is crucial for cellular defense against oxidative stress.
  • Nrf2 activity is regulated by Kelch-like ECH-associated protein 1 (Keap1), but Keap1 expression regulation is unclear.

Purpose of the Study:

  • To investigate the role of microRNA-7 (miR-7) in regulating Keap1 expression and the Nrf2 pathway.
  • To elucidate the cytoprotective mechanisms of miR-7 in neuronal cells.

Main Methods:

  • Investigated miR-7 targeting of Keap1 mRNA 3'-UTR in SH-SY5Y neuroblastoma cells.
  • Assessed Nrf2 activity by measuring its targets (HO-1, GCLM) and nuclear localization.
  • Evaluated oxidative stress markers (hydroperoxides, glutathione) and MPP+-induced toxicity in neuronal cells.

Main Results:

  • miR-7 directly represses Keap1 expression in human neuroblastoma cells.
  • miR-7 enhances Nrf2 activity, leading to increased expression of antioxidant genes and nuclear Nrf2.
  • miR-7 reduces oxidative stress and protects neuronal cells against MPP+-induced toxicity.

Conclusions:

  • miR-7 regulates the Nrf2-Keap1 pathway by targeting Keap1 mRNA.
  • miR-7 exerts cytoprotective effects against oxidative stress and neurotoxicity through Nrf2 pathway activation.