Related Experiment Video
Updated: Apr 1, 2026

A Rapid In Vivo Bioassay for Developmentally Active Enhancers
Neuropilin-2 genomic elements drive cre recombinase expression in primitive blood, vascular and neuronal lineages
Sophie Wiszniak1, Michaela Scherer1, Hayley Ramshaw1
1Centre for Cancer Biology and University of South Australia, Frome Road, Adelaide, South Australia, 5000, Australia.
Abstract:
We have established a novel Cre mouse line, using genomic elements encompassing the Nrp2 locus, present within a bacterial artificial chromosome clone. By crossing this Cre driver line to R26R LacZ reporter mice, we have documented the temporal expression and lineage traced tissues in which Cre is expressed. Nrp2-Cre drives expression in primitive blood cells arising from the yolk sac, venous and lymphatic endothelial cells, peripheral sensory ganglia, and the lung bud. This mouse line will provide a new tool to researchers wishing to study the development of various tissues and organs in which this Cre driver is expressed, as well as allow tissue-specific knockout of genes of interest to study protein function. This work also presents the first evidence for expression of Nrp2 protein in a mesodermal progenitor with restricted hematopoietic potential, which will significantly advance the study of primitive erythropoiesis. genesis 53:709-717, 2015. © 2015 Wiley Periodicals, Inc.
Related Concept Videos
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Non-LTR Retrotransposons
Neurulation

