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Immobilization of pectin depolymerising polygalacturonase using different polymers.

Haneef Ur Rehman1, Afsheen Aman2, Muhammad Asif Nawaz3

  • 1Department of Chemistry, University of Turbat, Kech, Balochistan, Pakistan.

International Journal of Biological Macromolecules
|October 11, 2015
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Summary

Immobilizing polygalacturonase using polyacrylamide gel enhanced its stability and activity. This enzyme immobilization technique offers improved reusability and thermal tolerance for industrial applications.

Keywords:
Agar-agar matrixCalcium alginate beadsEntrapmentImmobilizationPolyacrylamide gelPolygalacturonase

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Area of Science:

  • Biochemistry
  • Enzyme Technology

Background:

  • Polygalacturonase is crucial for pectin hydrolysis, with applications in food and textile industries.
  • Enzyme immobilization enhances stability, reusability, and catalytic efficiency.

Purpose of the Study:

  • To investigate polygalacturonase immobilization using different polymer matrices.
  • To evaluate the impact of immobilization on enzyme activity, stability, and operational parameters.

Main Methods:

  • Screening of calcium alginate beads, polyacrylamide gel, and agar-agar for polygalacturonase entrapment.
  • Assessing immobilization yield, enzyme activity, optimal reaction time, temperature, and pH.
  • Evaluating thermal stability and reusability of immobilized enzyme preparations.

Main Results:

  • Polyacrylamide gel yielded the highest immobilization (89%), surpassing agar-agar (80%) and calcium alginate (46%).
  • Immobilization extended optimal reaction time to 10 min and increased optimal temperatures.
  • Immobilized polygalacturonase exhibited enhanced thermal stability and retained over 80% activity after two cycles.

Conclusions:

  • Polyacrylamide gel is a highly effective matrix for polygalacturonase immobilization.
  • Immobilization significantly improves enzyme's operational stability, reusability, and thermal tolerance.
  • This study demonstrates a viable method for enhancing polygalacturonase utility in industrial processes.