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Related Experiment Video

Updated: Apr 1, 2026

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

A simple and versatile cell wall staining protocol to study plant reproduction.

Thomas J Musielak¹, Laura Schenkel¹, Martina Kolb¹

¹Department of Cell Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076, Tübingen, Germany.

Plant Reproduction

|October 12, 2015

Summary

SCRI Renaissance 2200 is a versatile dye for studying plant reproduction using confocal microscopy. This easy-to-use stain aids in visualizing pollen tube guidance and early embryo development in Arabidopsis thaliana.

Keywords:

Arabidopsis thaliana Cell wall staining Confocal microscopy Embryogenesis Pollen tube Renaissance SR2200

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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Published on: February 27, 2021

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Last Updated: Apr 1, 2026

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

Published on: June 19, 2014

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Published on: February 27, 2021

Area of Science:

Plant reproductive biology
Microscopy techniques
Cellular imaging

Background:

Traditional plant reproduction studies rely on light microscopy and staining.
Fluorescent proteins and confocal microscopy advanced visualization of plant reproduction.
Cell perimeter staining is crucial for contextualizing fluorescent signals.

Purpose of the Study:

To introduce SCRI Renaissance 2200 (SR2200) as a versatile dye.
To demonstrate SR2200's utility in studying plant reproduction via confocal microscopy.
To showcase SR2200's compatibility with fluorescent proteins and other dyes.

Main Methods:

Application of SR2200 dye for microscopic analysis.
Confocal laser scanning microscopy imaging.
Combination staining with fluorescent proteins and DAPI.

Main Results:

SR2200 effectively visualizes pollen tube growth, guidance, and reception.
SR2200 aids in studying early embryo patterning in Arabidopsis thaliana.
SR2200 is compatible with various fluorescent proteins and can be used for double labeling with dyes like DAPI.

Conclusions:

SCRI Renaissance 2200 is a versatile dye for plant reproduction research.
SR2200 offers an easy-to-use alternative to existing staining protocols.
This dye enhances microscopic analysis in plant reproductive biology.