Templated polymers enable selective capture and release of lysophosphatidic acid in human plasma via optimization of
Jialu Wang1, Martha Sibrian-Vazquez1, Jorge O Escobedo1
1Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
Abstract:
The first solid phase extraction materials for selective lysophosphatidic acid (LPA) enrichment from human plasma are described. Molecularly imprinted polymers were designed, synthesized and evaluated as cartridge fillings. They enabled a relatively rapid and simple extraction protocol for LPA without any need for multiple liquid-liquid extraction steps. The five major subspecies of lysophosphatidic acid are readily separated from all other native plasma phospholipids, including those well-known to interfere with LPA quantitation, such as phosphatidylcholine and lysophosphatidylcholine. Outstanding LPA purity is obtained via these solid phase materials in a tandem extraction setup.
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