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Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic
Mayeul Collot1, Rémy Kreder1, Anatoliy L Tatarets2
1Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401, Illkirch, France. andrey.klymchenko@unistra.fr mayeul.collot@unistra.fr.
Abstract:
A rational design of squaraine dyes with lipophilic and zwitterionic groups tunes cell entry, allowing for selective far-red/near-infrared imaging of plasma membrane vs. endoplasmic reticulum. They exhibit up to 110-fold fluorescence enhancement in biomembranes and enable cellular imaging at 1 nM concentration, which make them the brightest membrane probes to date.
