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Forensic Loci Allele Database (FLAD): Automatically generated, permanent identifiers for sequenced forensic alleles.

Christophe Van Neste1, Wim Van Criekinge2, Dieter Deforce1

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Forensic Science International. Genetics
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Summary

Massively parallel sequencing offers advantages in forensic genetics, but standardized reporting is lacking. The Forensic Loci Allele Database (FLAD) provides permanent identifiers for forensic alleles, aiding nomenclature and data sharing.

Keywords:
DatabaseForensic lociMPSNGSNomenclatureSTR

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Area of Science:

  • Forensic genetics
  • Bioinformatics
  • Molecular biology

Background:

  • Capillary electrophoresis is the current standard for forensic STR loci analysis.
  • Massively parallel sequencing (MPS) presents potential benefits like increased multiplexing and SNP detection for forensic applications.
  • A lack of consensus exists regarding the standardized reporting of MPS-generated forensic profiles.

Discussion:

  • The Forensic Loci Allele Database (FLAD) service offers a solution for standardized nomenclature of forensic alleles.
  • FLAD provides permanent, GenBank-style identifiers for short tandem repeat (STR) and single nucleotide polymorphism (SNP) alleles and their microvariants.
  • This database aims to facilitate consistent reporting and interpretation of MPS data in forensic genetics.

Key Insights:

  • FLAD establishes a centralized repository for unique forensic allele identifiers.
  • The service supports both STR and SNP loci, accommodating diverse forensic genetic analyses.
  • Permanent identifiers are crucial for inter-laboratory comparisons and legal admissibility of genetic evidence.

Outlook:

  • FLAD can support researchers developing new forensic sequencing kits and conducting population studies.
  • The freely available online service promotes data standardization and collaboration within the forensic genetics community.
  • Adoption of FLAD is expected to streamline the integration of MPS technologies into routine forensic casework.