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Related Concept Videos

Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Biosensor for label-free DNA quantification based on functionalized LPGs.

Helena M R Gonçalves1, Luis Moreira1, Leonor Pereira2

  • 1University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal.

Biosensors & Bioelectronics
|October 13, 2015
PubMed
Summary

This study introduces a label-free fiber optic biosensor for DNA hybridization detection. The novel sensor demonstrates high specificity, capable of identifying single base mismatches in DNA targets.

Keywords:
BiosensingDNADetectionLabel-freeQuantification

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Area of Science:

  • Biomedical Engineering
  • Optoelectronics
  • Molecular Biology

Background:

  • Label-free biosensing is crucial for real-time biomolecular detection.
  • Fiber optic sensors offer advantages in sensitivity and remote sensing capabilities.
  • Detecting DNA hybridization is fundamental in diagnostics and research.

Purpose of the Study:

  • To develop and validate a label-free fiber optic biosensor for in-situ DNA hybridization.
  • To propose a new methodology for determining the spectral position of long period grating (LPG) mode resonance.
  • To assess the sensor's limit of detection, quantification, specificity, and selectivity.

Main Methods:

  • Utilized a long period grating (LPG) fiber optic configuration.
  • Employed a basic optical interrogation scheme with off-the-shelf components.
  • Developed a novel method for LPG mode resonance spectral position determination.
  • Experimentally validated DNA detection using targets with varying base mismatches.

Main Results:

  • Achieved an experimental limit of detection (LOD) of 62±2 nM for DNA.
  • Determined a limit of quantification (LOQ) of 209±7 nM.
  • Demonstrated high specificity, successfully identifying single base mismatches in DNA targets.
  • Confirmed the sensor's capability for in-situ DNA hybridization detection.

Conclusions:

  • The developed label-free fiber optic biosensor is effective for sensitive and specific DNA hybridization detection.
  • The proposed methodology for LPG resonance determination enhances sensor performance.
  • The sensor's single base mismatch selectivity holds significant potential for diagnostic applications.
  • The use of off-the-shelf components suggests a cost-effective and practical biosensing solution.