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Related Experiment Videos

Successful allogeneic cryopreserved marrow transplantation.

L C Lasky1, N Van Buren, D J Weisdorf

  • 1Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis.

Transfusion
|February 1, 1989
PubMed
Summary

Successful engraftment was achieved in allogeneic bone marrow transplantation (BMT) using cryopreserved marrow. This study demonstrates the viability of frozen marrow for allogeneic BMT, offering hope for more patients.

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Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Cryopreservation is widely used in autologous bone marrow transplantation (BMT) but less so in allogeneic BMT.
  • Graft-versus-host disease is a significant concern in allogeneic BMT.

Purpose of the Study:

  • To evaluate the efficacy of cryopreserved marrow in allogeneic BMT.
  • To assess engraftment success and long-term outcomes in patients receiving allogeneic BMT with frozen marrow.

Main Methods:

  • Six patients with various hematologic conditions underwent allogeneic BMT using cryopreserved marrow.
  • Marrow was collected, cryopreserved using controlled-rate freezing with 10% DMSO in liquid nitrogen, and stored.
  • T-cells were removed in one case to mitigate graft-versus-host disease.

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Main Results:

  • Successful engraftment, defined as WBC > 1000/uL for 3 days, was observed in all cases between 13 and 37 days post-transplant.
  • Donor origin of engraftment was confirmed using cytogenetics, red cell typing, and restriction fragment length polymorphisms.
  • Three patients remain alive and well at 21, 21, and 42 months post-BMT.

Conclusions:

  • Cryopreserved marrow can be successfully utilized for allogeneic BMT.
  • This approach offers a viable option for patients requiring allogeneic hematopoietic stem cell transplantation.