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Differential Scanning Calorimetry &#8212; A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
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Formulation screening by differential scanning fluorimetry: how often does it work?

Marko Ristic1, Nicholas Rosa1, Shane A Seabrook1

  • 1Manufacturing Flagship, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.

Acta Crystallographica. Section F, Structural Biology Communications
|October 13, 2015
PubMed
Summary
This summary is machine-generated.

Screening protein formulations using differential scanning fluorimetry (DSF) significantly improved protein stability in over 35% of cases. Citrate, bis-tris, and ADA buffers were identified as particularly effective for protein crystallization.

Keywords:
Thermofluorbufferdifferential scanning fluorimetryformulationprotein stability

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Area of Science:

  • Biochemistry
  • Protein Crystallization
  • Formulation Science

Background:

  • Protein sample quality (folding and uniformity) is critical for successful protein crystallization.
  • Formulation chemistry profoundly impacts protein sample behavior and stability.
  • Differential scanning fluorimetry (DSF) is an accessible technique for assessing protein stability under varying conditions.

Purpose of the Study:

  • To investigate the effect of formulation screening on protein stability using DSF.
  • To identify optimal buffer formulations for enhancing protein stability.
  • To evaluate the utility of standard buffer formulations in protein sample preparation.

Main Methods:

  • A diverse set of 252 soluble protein samples was analyzed.
  • A standard formulation-screening protocol employing DSF was applied.
  • Automated analysis was used to assess protein stability across different formulations.

Main Results:

  • Buffer screening led to a significant increase in protein stability in over 35% of tested samples.
  • Three specific formulations demonstrated superior performance in enhancing protein stability.
  • Citrate, bis-tris, and ADA buffers were identified as highly effective for protein formulations.

Conclusions:

  • Formulation screening is a crucial step for optimizing protein stability for crystallization.
  • Specific buffers, including citrate, bis-tris, and ADA, are highly recommended for protein formulation.
  • DSF provides an efficient method for identifying stabilizing buffer conditions for proteins.