Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exact integrated equations to describe diffusion kinetics.

Soft matter·2026

Same author

Diffusion kinetics of volatile organic compounds monitored by nanohole surface plasmonics.

The Analyst·2026

Same author

Long-range strain sensing system using a frequency-locked π-shifted grating.

Optics express·2025

Same author

Mineralogical Analysis of Solid-Sample Flame Emission Spectra by Machine Learning.

Analytical chemistry·2024

Same author

Coaxial burner system for solid-sample flame emission spectroscopy.

Analytical methods : advancing methods and applications·2024

Same author

Estimation of effective number of breeders and effective population size in an abundant and heavily exploited marine teleost.

Evolutionary applications·2024

Same journal

Nanopore sequencing with proteins: synchronization and dischronization of molecular dynamics simulations with laboratory and industrial developments.

Soft matter·2026

Same journal

Catanionics from biosurfactants and regular surfactants: miscibility and structure.

Soft matter·2026

Same journal

Adhesives with a thickness smaller than the fractocohesive length enhance adhesion.

Soft matter·2026

Same journal

Non-equilibrium phase transitions in hybrid Voronoi models of cell colonies.

Soft matter·2026

Same journal

Effects of methoxy substituents on self-assembly and gelation performance of benzamide-based organogelators.

Soft matter·2026

Same journal

Rheology of <i>Escherichia coli</i> suspensions with various bacterial morphologies and motion characteristics.

Soft matter·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

Quantitative diffusion and swelling kinetic measurements using large-angle interferometric refractometry.

John E Saunders¹, Hao Chen¹, Chris Brauer¹

¹Department of Chemistry, Queen's University, Kingston, Ontario, Canada. hploock@queensu.ca.

|October 13, 2015

Summary

This summary is machine-generated.

A new model accurately calculates sorbate concentration in films using refractive index and thickness. This method, validated with water uptake in SU-8 films, reveals two Fickian diffusion processes.

More Related Videos

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

Related Experiment Videos

Last Updated: Apr 1, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels

Published on: September 8, 2016

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

Area of Science:

Materials Science
Physical Chemistry
Polymer Science

Background:

Sorbate uptake and release alter film properties like refractive index and thickness.
Accurate quantification of sorbate concentration is crucial for understanding film behavior.

Purpose of the Study:

To develop and validate a comprehensive model for calculating sorbate concentration from film refractive index and thickness.
To investigate the uptake kinetics of solvents, specifically water, into SU-8 photoresist films.

Main Methods:

Utilized Lorentz-Lorenz and Clausius-Mosotti equations to model sorbate partitioning.
Employed large-angle interferometric refractometry for film characterization (15-150 μm thickness).
Applied Fourier analysis for real-time determination of film thickness and refractive indices.

Main Results:

The model successfully correlates average refractive index and film thickness with sorbate mass fraction.
Water uptake into SU-8 films exhibited two distinct Fickian diffusion processes.
Calculated diffusion coefficients for water in SU-8 were 5.67 × 10⁻⁹ cm²/s and 61.2 × 10⁻⁹ cm²/s.

Conclusions:

The developed model provides a quantitative method for determining sorbate concentration in films.
The study elucidates the complex diffusion kinetics of water in SU-8 photoresist.
Interferometric refractometry coupled with Fourier analysis is effective for dynamic film analysis.