Related Experiment Video
Updated: Apr 1, 2026
Controlled Synthesis and Fluorescence Tracking of Highly Uniform PolyN-isopropylacrylamide Microgels
Published on: September 8, 2016
Dosimetric properties of N-isopropylacrylamide polymer gel using nonelectrophoresis grade BIS in preparation
Roghayeh Khodadadi, Azim Khajeali, Ali Reza Farajollahi1
1Medical Education Research Center; Department of Medical Physics, Faculty of Medicine; Imam Reza Hospital, Radiotherapy Department, Tabriz University of Medical Sciences, Tabriz, Iran.
Introduction:
Unlike availability of the different grades of N, N'-methylenebisacrylamide (BIS), electrophoresis is recommended in literature as a crosslinking agent in gel preparation. As the cost of non-electrophoresis grade BIS is much less than that of electrophoresis, the dosimetric characteristics of the of the polymer gel using non-electrophoresis BIS is evaluated in terms of photon energy and dose rate.
Materials And Methods:
To compare the response of this gel with the one that contains electrophoresis grade BIS, two sets of N-isopropylacrylamide (NIPAM) gel were prepared using electrophoresis and non-electrophoresis BIS and irradiated to different gamma doses.
Results:
It was shown that the dose-response of NIPAM gel made from non-electrophoresis grade BIS is coincident to that of electrophoresis grade BIS. The study of dose response of NIPAM with non-electrophoresis grade BIS as a function of beam energy revealed no dependence on radiation energies of 1.25 MV from 60 Co and 6 MV and 18MV from linear accelerator. It was found that dose rate has no influence on dose response of NIPAM gel with non-electrophoresis BIS.
Conclusion:
Substitution non-electrophoresis grade BIS not only reduces the cost of gel preparation without any adverse effect on its dose response, but also its lower background increases the dynamic range of dose linearity.
More Related Videos
09:09Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
Published on: February 27, 2016
07:38DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
Published on: October 6, 2017
Related Concept Videos
01:27SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
01:22Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
02:35DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...