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Point-of-Care Quantitative Measure of Glucose-6-Phosphate Dehydrogenase Enzyme Deficiency

Vinod K Bhutani1, Michael Kaplan2, Bertil Glader3

  • 1Department of Pediatrics, Stanford University School of Medicine, Stanford, California; bhutani@stanford.edu.

Pediatrics
|October 14, 2015
PubMed

Insights

A new digital microfluidics platform accurately screens for glucose-6-phosphate dehydrogenase (G6PD) deficiency in newborns. This point-of-care test could prevent severe newborn complications.

Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Point-of-Care Testing

Background:

  • Glucose-6-phosphate dehydrogenase (G6PD) deficiency affects newborns, potentially causing bilirubin neurotoxicity.
  • Early screening is crucial for preventing severe neonatal complications.

Purpose of the Study:

  • To evaluate a quantitative G6PD assay on a digital microfluidic platform.
  • To compare its performance against standard clinical methods for newborn screening.

Main Methods:

  • Quantitative G6PD activity measurement using digital microfluidic fluorescence.
  • Comparison with the gold standard fluorescence biochemical test.
  • Analysis of 98 discarded blood samples, including 24 G6PD-deficient samples.

Main Results:

  • Digital microfluidics showed comparable results to the standard method for both normal and G6PD-deficient samples.
  • Bland-Altman analysis indicated a mean difference of -0.96 ± 1.8 U/g Hb.
  • The assay effectively discriminated between G6PD-deficient and normal samples with no overlap.

Conclusions:

  • The digital microfluidics platform demonstrates potential as an accurate point-of-care tool.
  • Further validation is recommended for rapid newborn G6PD screening.
  • This technology could significantly improve newborn health outcomes by enabling early detection.
Abstract

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