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L-fucose blocks MIF/MAF priming of rabbit alveolar macrophages for a PMA-induced oxidative response
K Umehara1, H Hayakawa, Q N Myrvik
1Department of Microbiology and Immunology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.
Abstract:
The effect of L-fucose on the priming of AM from normal adult rabbits and their subsequent chemiluminescent (CL) responses to phorbol myristate acetate (PMA) was investigated. It was observed that 12.5 mM L-fucose, but not D-fucose, blocked the "spontaneous" priming of normal AM during an 18-hr incubation period in serum-free RPMI 1640 medium by about 40% (P less than 0.01) as detected by their CL responses to a PMA challenge. In addition, the optimal concentration of L-fucose (12.5 mM) blocked MIF/MAF priming during the 3- or 18-hr incubation period by 71 or 93% (P less than 0.05), respectively, as determined by their CL responses following PMA challenge. It is of particular significance that D-fucose was inactive. These results, together with previously published data, indicate that L-fucose (a) blocks priming of AM for an oxidative response, (b) stimulates random migration of AM, and (c) reverses migration inhibition produced by migration inhibition factor.
Insights
L-fucose inhibits the priming of alveolar macrophages (AM) for oxidative responses. This sugar also stimulates random migration and reverses migration inhibition, unlike D-fucose.
Area of Science:
- Immunology
- Cell Biology
Background:
- Alveolar macrophages (AM) play a critical role in immune responses.
- Priming enhances the responsiveness of AM to stimuli.
- Chemiluminescence (CL) is a measure of oxidative burst activity in phagocytes.
Purpose of the Study:
- To investigate the effect of L-fucose on AM priming.
- To assess the impact of L-fucose on subsequent chemiluminescent responses to phorbol myristate acetate (PMA).
Main Methods:
- Incubation of normal adult rabbit AM with L-fucose or D-fucose in serum-free RPMI 1640 medium.
- Measurement of spontaneous and MIF/MAF-induced AM priming.
- Assessment of CL responses to PMA challenge to quantify oxidative burst.
Main Results:
- 12.5 mM L-fucose significantly blocked spontaneous AM priming by approximately 40% (P < 0.01).
- L-fucose (12.5 mM) blocked MIF/MAF-induced priming by 71% (3-hr) and 93% (18-hr) (P < 0.05).
- D-fucose showed no inhibitory effect on AM priming.
Conclusions:
- L-fucose effectively blocks the priming of AM for oxidative responses.
- L-fucose stimulates random migration of AM.
- L-fucose can reverse migration inhibition induced by migration inhibition factor.
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