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Down-Regulating Receptor Interacting Protein Kinase 1 (RIP1) Promotes Oxaliplatin-Induced Tca8113 Cell Apoptosis

BaoZhong Shan1, Feng Ma2, MingGuo Wang2

  • 1School of Stomatology, Shandong University, Jinan, Shandong, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|October 14, 2015
PubMed
Abstract

Insights

Oxaliplatin chemotherapy inhibits tongue cancer cell growth by reducing mitochondrial potential and activating caspase-3. Down-regulating receptor-interacting protein kinase 1 (RIP1) enhances oxaliplatin-induced apoptosis in these cancer cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Oxaliplatin is a key chemotherapy agent for colorectal and oral cancers.
  • The precise mechanism of oxaliplatin's action on tongue squamous cell carcinoma remains unclear.

Purpose of the Study:

  • To elucidate the molecular mechanism by which oxaliplatin affects the survival and death of tongue squamous cell carcinoma Tca8113 cells.

Main Methods:

  • Tca8113 cells were treated with oxaliplatin (1 μmol/L for 24 h).
  • Cell proliferation, apoptosis, and mitochondrial membrane potential were assessed.
  • Western blot was used to analyze receptor-interacting protein kinase 1 (RIP1) expression.
  • The effect of RIP1 knockdown via siRNA on oxaliplatin-induced apoptosis was investigated.

Main Results:

  • Oxaliplatin significantly inhibited Tca8113 cell growth, reduced mitochondrial membrane potential, and induced phosphatidylserine eversion.
  • Caspase-3 activation was observed, and RIP1 expression increased following oxaliplatin treatment.
  • Knockdown of RIP1 using siRNA potentiated oxaliplatin-induced apoptosis in Tca8113 cells.

Conclusions:

  • Down-regulation of RIP1 promotes oxaliplatin-induced apoptosis in Tca8113 tongue squamous cell carcinoma cells.
  • RIP1 is implicated in the cellular response to oxaliplatin treatment in this cancer model.

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