Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K
The Proteasome01:13

The Proteasome

2.0K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
2.0K
The Proteasome02:18

The Proteasome

10.5K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.5K
The Proteasome02:18

The Proteasome

5.1K
5.1K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

4.7K
4.7K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

14.9K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Proteomic Analysis of Cerebrospinal Fluid From Neurological Patients With and Without Amyotrophic Lateral Sclerosis.

Journal of neurochemistry·2026
Same author

Myosin Post-Translational Modifications Associated With Critical Illness Myopathy.

Acta physiologica (Oxford, England)·2026
Same author

Systems neuroendocrinology in ME/CFS and long COVID: a chronobiological framework for hormone-based research.

Frontiers in neuroendocrinology·2026
Same author

Combatting ventilator induced diaphragm dysfunction with human bone marrow mesenchymal stromal cell-derived extracellular vesicles.

Skeletal muscle·2026
Same author

Designing studies for post-treatment Lyme disease and other infection-associated chronic illnesses.

Brain : a journal of neurology·2026
Same author

Proteomic signatures in cerebrospinal fluid and their clinical associations in patients with ME/CFS.

Scientific reports·2026

Related Experiment Video

Updated: Apr 1, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

13.6K

Proteomics to Understand the Degenerative Matter.

Jonas Bergquist1

  • 1BMC and SciLife Lab, Uppsala University (Department of Chemistry), Analytical Chemistry, Sweden.

Free Radical Biology & Medicine
|October 14, 2015
PubMed
Summary

Aging and disease cause loss of bodily control. This study identifies protein biomarkers in brain and muscle using shotgun proteomics and dimethyl labeling (DML) to understand degeneration.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Gerontology

Background:

  • Human aging and disease inevitably lead to loss of bodily functions, muscle control, and innervation.
  • Degenerative effects can result from natural aging, progressive diseases, or traumatic injuries.
  • Understanding these degenerative processes is crucial for developing interventions.

Purpose of the Study:

  • To identify protein biomarkers associated with degenerative processes in the human brain and skeletal muscle.
  • To characterize structural modifications of proteins involved in disease-related degeneration.
  • To gain insights into the molecular mechanisms underlying natural aging and age-related diseases.

Main Methods:

  • Utilized a shotgun proteomic approach for global proteome analysis.

More Related Videos

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches
09:47

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches

Published on: December 15, 2023

2.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K

Related Experiment Videos

Last Updated: Apr 1, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

13.6K
Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches
09:47

Author Spotlight: Advancing Alzheimer's Research – Exploring Early Detection and Multi-Omics Approaches

Published on: December 15, 2023

2.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.9K
  • Employed relative quantitative dimethyl labeling (DML) for precise protein quantification.
  • Analyzed limited biological material, such as tissue biopsies.

    • Main Results:

    • Successfully analyzed global changes in the human brain and skeletal muscle proteome.
    • Identified key proteins and their modifications implicated in degenerative conditions.
    • Discovered novel factors contributing to the aging process and functional decline.

    Conclusions:

    • Shotgun proteomics with DML is effective for analyzing proteome changes in degeneration with minimal sample requirements.
    • Identified protein biomarkers can aid in early detection and understanding of neurodegenerative and muscle-wasting diseases.
    • The study provides new knowledge on the molecular underpinnings of aging and disease-induced degeneration.