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Expression of ricin B chain in Escherichia coli
K Hussain1, C Bowler, L M Roberts
1Department of Biological Sciences, University of Warwick, Coventry, England.
FEBS Letters
|February 27, 1989
Abstract:
DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.