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[Detection of microRNAs seed sequences within human papillomavirus genomes]

David Pineda-Gómez1, Efraín Garrido, Pedro Chávez

  • 1Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Distrito Federal, México. maosal89@yahoo.com.

Revista Medica Del Instituto Mexicano Del Seguro Social
|October 15, 2015
PubMed

Insights

This study discovered human and viral microRNA seed sequences within high and low-risk human papillomavirus (HPV) genomes, suggesting a shared viral gene regulation mechanism. These oncogenic sequences were identified using in silico analysis.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Human papillomavirus (HPV) is a common viral infection linked to cancer.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression.
  • The presence and role of viral miRNAs within HPV genomes remain incompletely understood.

Purpose of the Study:

  • To investigate the presence of human and viral microRNA seed sequences within high and low-risk HPV genomes.
  • To identify potential oncogenic microRNA sequences and their genomic locations.
  • To explore a potential common viral mechanism for gene expression regulation.

Main Methods:

  • In silico analysis of HPV genomes (high-risk types 16, 18, 33, 45, 52 and low-risk types 6, 11).
  • Utilized microRNA sequences from Sanger's database for comparison.
  • Focused on identifying seed sequences and specific microRNA fingerprints, particularly miR-297 and miR-363.

Main Results:

  • Detected seed sequences of human and viral microRNAs in both high and low-risk HPV genomes.
  • Identified a potential fingerprint with repeated miR-297 sequences in the LCR region of several high-risk HPV types.
  • Observed absence of the miR-297 fingerprint in HPV11, though other sequences like miR-363 were present.

Conclusions:

  • HPV genomes harbor microRNA sequences with high oncogenic potential.
  • A specific miR-297 fingerprint in high-risk HPV types suggests a targeted viral mechanism.
  • These findings point towards a conserved, putative viral mechanism for gene expression regulation across different human viruses.
Keywords:
HPVMicroRNAs

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