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WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1

Long Lv1, Gong Chen2, Jianping Zhou3

  • 1Department of General Surgery, People's Hospital of Gaochun, No. 9 Chunzhong Road, Gaochun, Nanjing, 211300, Jiangsu Province, China. l3851596501@163.com.

Journal of Experimental & Clinical Cancer Research : CR
|October 15, 2015
PubMed
Abstract

Insights

The long non-coding RNA WT1-antisense (WT1-AS) acts as a tumor suppressor in hepatocellular carcinoma (HCC). WT1-AS downregulates WT1 expression, promoting apoptosis and improving prognosis in HCC patients.

Area of Science:

  • Molecular biology
  • Oncology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a significant global health concern.
  • The role of the WT1-antisense (WT1-AS) long non-coding RNA in HCC development is largely unknown.
  • WT1 is implicated in various cancers, but its specific role in HCC requires further investigation.

Purpose of the Study:

  • To elucidate the function of WT1-AS in hepatocellular carcinoma (HCC).
  • To investigate the regulatory relationship between WT1-AS and the WT1 gene in HCC.
  • To determine the prognostic value of WT1-AS in HCC patients.

Main Methods:

  • Quantitative real-time PCR and western blot to assess WT1-AS and WT1 expression levels.
  • EdU incorporation and PI-Annexin-V staining to evaluate cell proliferation and apoptosis.
  • ShRNA and dual-luciferase assays to explore the WT1-AS/WT1 regulatory axis.
  • Bioinformatics and Kaplan-Meier analyses for prognostic assessment.

Main Results:

  • WT1-AS expression was inversely correlated with WT1 expression in HCC tissues.
  • Downregulation of WT1 by WT1-AS suppressed the JAK/STAT3 pathway, promoting apoptosis.
  • WT1-AS binds to the WT1 promoter region, downregulating WT1 expression.
  • WT1-AS expression serves as a favorable prognostic indicator for HCC patients.
  • WT1-AS reversed Doxorubicin resistance in HCC cells.

Conclusions:

  • WT1-AS functions as a tumor suppressor in HCC by downregulating WT1 expression.
  • WT1-AS promotes apoptosis and may reverse oncogenic effects of WT1 in HCC.
  • WT1-AS represents a potential therapeutic target for improving HCC prognosis.

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