Related Experiment Video
Updated: Apr 1, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
Novel Fluorescein-Based Fluorophores: Synthesis, Photophysics and Micro-Environmental Study
Rahul Telore1, Santosh Chemate1, Vikas Padalkar1
1Dyestuff Technology Department, N. P. Marg, Tinctorial Chemistry Group, Institute of Chemical TechnologyMatunga, Mumbai, 400 019, India.
Abstract:
Carboxylic acid substituted fluorescein derivatives were synthesized from 2,4-dihydroxy carboxylic acid and phthalic anhydride or trimellitic anhydride. The photophysical properties like absorption, emission and fluorescence quantum yields of these fluorophores were studied. The conjugation studies of these fluorophores with different proteins at different concentrations were carried out. The effect of pH on photophysical properties was studied at different pH ranges from 5 to 12. The change in the absorption and emission with respect to viscosity of medium was also studied. The changes in the electronic transition, energy levels, and orbital diagram of the fluorescein derivatives were computed using the TD-DFT computations and co-related with experimental photophysical data.
Related Concept Videos
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Applications
Super-resolution Fluorescence Microscopy

