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Updated: Apr 1, 2026

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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
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Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation
Yuan Cao1, Giang K T Nguyen1, James P Tam1
1School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.
Abstract:
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.

