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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Related Experiment Video

Updated: Apr 1, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Developing a matrix reference material for screening of transgenic rice.

Jun Li1,2, Yuhua Wu1,2, Xiaofei Li1,2

  • 1Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan, 430062, China.

Analytical and Bioanalytical Chemistry
|October 15, 2015
PubMed
Summary
This summary is machine-generated.

This study developed a novel transgenic rice reference material (SDrice) for screening genetically modified organisms (GMOs). SDrice DNA enables accurate GMO screening and PCR assay validation, addressing the lack of suitable GMO certified reference materials.

Keywords:
ComparabilityMatrix RMPositive controlScreeningTransgenic rice

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Area of Science:

  • Food Science and Technology
  • Biotechnology
  • Molecular Biology

Background:

  • Detection of genetically modified organisms (GMOs) in food and feed relies on screening methods.
  • A significant challenge in GMO detection is the scarcity of compatible certified reference materials (CRMs).
  • Existing reference materials like plasmid DNA have limitations for screening applications.

Purpose of the Study:

  • To develop and validate a transgenic rice material as a CRM for GMO screening.
  • To assess the suitability of the developed material for determining the sensitivity and limit of detection (LOD) of PCR assays.
  • To overcome the drawbacks associated with using plasmid DNA as a reference material.

Main Methods:

  • Development of transgenic rice lines using a construct with 11 common screening elements.
  • Identification of a homozygous single-copy line (SDrice) using digital PCR.
  • Qualitative and quantitative assessment of SDrice DNA in PCR and real-time PCR assays.

Main Results:

  • SDrice genomic DNA was confirmed suitable as a positive control for screening GMO ingredients.
  • Real-time PCR assays using SDrice DNA as calibrators showed good linearity and LODs of 2-5 copies.
  • SDrice demonstrated significant differences compared to other GM rice events, indicating its unsuitability for quantifying other GM rice events.

Conclusions:

  • A novel matrix reference material (RM) suitable for screening GM rice has been successfully developed.
  • SDrice facilitates the determination of sensitivity and LOD for PCR-based GMO detection assays.
  • This study provides a valuable alternative to plasmid DNA, enhancing the reliability of GMO screening.