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Dissemination of fusidic acid resistance among Staphylococcus aureus clinical isolates

Fangyou Yu1, Yunling Liu2, Chaohui Lu3

  • 1Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. wzjxyfy@163.com.

BMC Microbiology
|October 15, 2015
PubMed
Abstract

Insights

Fusidic acid resistance in Staphylococcus aureus is increasing, particularly in China. The fusB gene and the ST5-MRSA-II-t2460 clone are driving this concerning trend in clinical isolates.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Fusidic acid (FA) resistance in Staphylococcus aureus is a growing concern, especially with prolonged use.
  • Investigating the prevalence and mechanisms of FA resistance in clinical S. aureus isolates is crucial.

Purpose of the Study:

  • To determine the dissemination and molecular characteristics of fusidic acid resistance among clinical Staphylococcus aureus isolates in China.
  • To identify the primary genetic determinants and clonal types associated with FA resistance.

Main Methods:

  • Antimicrobial susceptibility testing using disc-diffusion and agar dilution methods.
  • Detection of FA resistance genes (e.g., fusB) via PCR and DNA sequencing.
  • Molecular typing including SCCmec, spa, and multi-locus sequence typing (MLST) for clonal analysis.

Main Results:

  • 14.3% of 392 S. aureus isolates were resistant to FA, with higher rates in MRSA (27.1%) than MSSA (3.3%).
  • FA resistance significantly increased from 2012 to 2013, with the fusB gene found in 73.2% of resistant isolates.
  • The ST5-MRSA-II-t2460 clone, belonging to CC5, was predominant among FA-resistant MRSA and overall FA-resistant S. aureus isolates.

Conclusions:

  • A significant increase in fusidic acid resistance among S. aureus in China was observed.
  • The fusB gene is the primary determinant of FA resistance, and the CC5 clone, particularly ST5-MRSA-II-t2460, is responsible for its spread.

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