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Tools and Principles for Microbial Gene Circuit Engineering.

Robert W Bradley1, Martin Buck2, Baojun Wang3

  • 1Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom; School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, United Kingdom.

Journal of Molecular Biology
|October 15, 2015
PubMed
Summary
This summary is machine-generated.

Synthetic biologists engineer microbes with novel genetic circuits for complex computation. This work reviews tools and design principles for robust synthetic gene circuit construction and optimization in microbes.

Keywords:
engineering toolsgene circuitmodularity and orthogonalitysynthetic biologytranscriptional and translational control

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Area of Science:

  • Synthetic biology
  • Genetic engineering
  • Microbial biotechnology

Background:

  • Natural biological systems exhibit complex signal processing.
  • Engineered microbes offer potential for sophisticated computation and control.
  • Advancements in synthetic gene circuits require predictive design and modular genetic parts.

Purpose of the Study:

  • To provide an overview of components and tools for engineering microbial gene circuits.
  • To introduce design principles for robust and scalable circuit performance.
  • To describe strategies for circuit failure analysis and fine-tuning.

Main Methods:

  • Review of versatile components and tools for microbial gene circuit engineering.
  • Highlighting recently developed RNA-based tools with large dynamic ranges.
  • Introduction of design principles for circuit insulation and context independence.
  • Description of strategies for failure identification, correction, and fine-tuning.

Main Results:

  • Availability of diverse components and tools for synthetic gene circuit construction.
  • Emergence of programmable RNA-based tools for enhanced circuit performance.
  • Established design principles for achieving robust and scalable gene circuits.
  • Efficient strategies for troubleshooting and optimizing synthetic gene circuits.

Conclusions:

  • Engineering complex synthetic gene circuits in microbes is advancing.
  • Modular and orthogonal genetic parts are crucial for successful circuit design.
  • RNA-based tools and robust design principles enhance circuit predictability and performance.
  • Systematic approaches facilitate the development and optimization of synthetic gene circuits.