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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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Related Experiment Video

Updated: Mar 31, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray.

Qiang Rui1, Zipeng Xu1, Peng Yang1

  • 1The Second Affiliated Hospital of Nanjing Medical University, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|October 15, 2015
PubMed
Summary
This summary is machine-generated.

Colorectal cancer (CRC) metastasis involves altered long noncoding RNA (lncRNA) expression in lymph nodes. This study identified over 1000 dysregulated lncRNA transcripts in metastatic lymph nodes compared to normal ones.

Keywords:
Colorectal cancerLong noncoding RNALymph node metastasisMicroarray

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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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Area of Science:

  • Genomics
  • Molecular Biology
  • Oncology

Background:

  • Colorectal cancer (CRC) is a significant global health concern.
  • Lymph node metastasis is a critical factor in CRC progression and patient prognosis.
  • Long noncoding RNAs (lncRNAs) are emerging as key regulators in various cancers, including CRC.

Purpose of the Study:

  • To comprehensively profile lncRNA expression patterns in normal lymph nodes (NLN) versus metastatic lymph nodes (MLN) in colorectal cancer patients.
  • To identify differentially expressed lncRNAs associated with lymph node metastasis in CRC.
  • To explore the potential functional roles of these dysregulated lncRNAs in CRC pathogenesis.

Main Methods:

  • lncRNA and mRNA expression profiling was performed using microarray analysis on paired MLN and NLN samples from three CRC patients.
  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to validate the expression of selected differentially expressed lncRNAs in an independent cohort of 26 patients.
  • Bioinformatic analysis, including coexpressed mRNA annotation, was used to predict the potential functions of identified lncRNAs.

Main Results:

  • A total of 1133 lncRNA transcripts exhibited significant differential expression between MLN and NLN in CRC.
  • The expression levels of three selected lncRNAs were successfully validated by qRT-PCR, confirming their dysregulation in metastatic lymph nodes.
  • Functional predictions indicated that several lncRNA groups may be involved in CRC-related biological pathways through cis- and/or trans-regulation of protein-coding genes.

Conclusions:

  • This study presents the first comprehensive report on lncRNA expression profiles in human MLN and NLN of colorectal cancer.
  • The findings reveal a substantial number of dysregulated lncRNA transcripts (over 1000) in metastatic lymph nodes.
  • These dysregulated lncRNAs represent potential novel biomarkers and therapeutic targets for colorectal cancer metastasis.