Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Long-term repetitive transcranial direct current stimulation in patients with disorders of consciousness: a preliminary study.

Brain injury·2024

Same author

Clinicopathological features, treatment modalities, and prognosis of esophageal neuroendocrine carcinoma: A single-center retrospective study.

Journal of digestive diseases·2023

Same author

A molecular paradigm: "Plug-and-play" chemical sensing and crypto-steganography based on molecular recognition and selective response.

Biosensors & bioelectronics·2022

Same author

Microwave-Assisted Synthesis of Silver Nanoparticles for Multimode Colorimetric Sensing of Multiplex Metal Ions and Molecular Informatization Applications.

ACS applied materials & interfaces·2022

Same author

Complete nucleotide sequence of a novel partitivirus infecting the plant-pathogenic fungus Phomopsis vexans.

Archives of virology·2020

Same author

Matter, energy and information network of a graphene-peptide-based fluorescent sensing system for molecular logic computing, detection and imaging of cancer stem cell marker CD133 in cells and tumor tissues.

The Analyst·2019

Search research articles

Related Experiment Video

Updated: Mar 31, 2026

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Published on: December 11, 2012

Flexibility Analysis of Bacillus thuringiensis Cry1Aa.

Xin Min Zhao¹, Li Qiu Xia², Xiao Ping Yang³

¹Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, Hunan, China; Key Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China.

Biomedical and Environmental Sciences : BES

|October 15, 2015

Summary

Bacillus thuringiensis toxin Cry1Aa shows decreased rigidity with increasing temperature, with key flexible regions identified in domain II. This study reveals critical insights into Cry1Aa protein dynamics.

Keywords:

Bacillus thuringiensis Constraint Network Analysis Cry1Aa Flexibility NMSim

More Related Videos

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Published on: August 23, 2018

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

Related Experiment Videos

Last Updated: Mar 31, 2026

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Published on: December 11, 2012

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Published on: August 23, 2018

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

Area of Science:

Structural biology
Biophysics
Molecular dynamics

Background:

Bacillus thuringiensis (Bt) toxins are crucial insecticidal proteins.
Understanding the structural dynamics of Bt toxins like Cry1Aa is vital for their application.
Protein flexibility influences biological activity and stability.

Purpose of the Study:

To investigate the flexibility and mobility of the Bacillus thuringiensis toxin Cry1Aa.
To identify regions of high and low flexibility within the Cry1Aa structure.
To understand how temperature affects the rigidity of Cry1Aa.

Main Methods:

Utilized graph theory-based Constraint Network Analysis (CNA).
Employed normal mode-based program NMsim.
Analyzed global and local flexibility indices and individual residue fluctuations.

Main Results:

Cry1Aa network rigidity decreased with increasing temperature.
Identified two phase transition points indicating structural rigidity loss.
Found rigid clusters in domains I and II, with flexible regions in all domains.
Pinpointed weak spots in C-terminal domain III and highest fluctuation in domain II's apical loop2.

Conclusions:

The Bacillus thuringiensis toxin Cry1Aa exhibits temperature-dependent flexibility.
Apical loops of domain II represent the most flexible regions.
Structural flexibility is distributed across all three domains, with specific vulnerable areas.