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Alu element-containing RNAs maintain nucleolar structure and function.

Maïwen Caudron-Herger1, Teresa Pankert2, Jeanette Seiler3

  • 1Genome Organization & Function, German Cancer Research Center (DKFZ) Bioquant Center, Heidelberg, Germany m.caudron@dkfz.de karsten.rippe@dkfz.de.

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|October 15, 2015
PubMed
Summary
This summary is machine-generated.

Intronic Alu RNAs (aluRNAs) organize the nucleus and are crucial for nucleolar structure and function. These non-coding RNAs link RNA polymerase I and II activities, impacting rRNA production.

Keywords:
Alu repeat‐containing RNARNA‐dependent phase separationnucleolus structure and function

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genomics

Background:

  • Non-coding RNAs are essential for nuclear organization.
  • The nucleolus is a key nuclear subcompartment involved in ribosome biogenesis.

Purpose of the Study:

  • To investigate the role of intronic Alu RNAs (aluRNAs) in nucleolar organization and function.
  • To elucidate the mechanism by which aluRNAs influence nucleolus integrity and rRNA synthesis.

Main Methods:

  • Fluorescence microscopy
  • RNA deep-sequencing
  • Antisense oligonucleotide-mediated depletion
  • Drug-induced inhibition of RNA polymerase II
  • Overexpression of aluRNA sequences

Main Results:

  • AluRNAs were found to be enriched in the nucleolus.
  • Depletion of aluRNAs disrupted nucleolar structure and impaired rRNA gene transcription.
  • Overexpression of aluRNA increased nucleolus size and pre-ribosomal RNA (pre-rRNA) levels.
  • AluRNAs interact with nucleolin and target genomic loci to the nucleolus.

Conclusions:

  • AluRNAs play a critical role in maintaining nucleolar structure and function.
  • A novel mechanism linking RNA polymerase I and II activities via aluRNAs modulates nucleolar function and rRNA production.