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Updated: Mar 31, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Published on: October 6, 2019
Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional
Liisa D van Vliet1, Pierre-Yves Colin1, Florian Hollfelder1
1Department of Biochemistry , University of Cambridge , 80 Tennis Court Road, Cambridge CB2 1GA , UK.
Abstract:
The idea of compartmentalization of genotype and phenotype in cells is key for enabling Darwinian evolution. This contribution describes bioinspired systems that use in vitro compartments-water-in-oil droplets and gel-shell beads-for the directed evolution of functional proteins. Technologies based on these principles promise to provide easier access to protein-based therapeutics, reagents for processes involving enzyme catalysis, parts for synthetic biology and materials with biological components.
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