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Secondary bile acids effects in colon pathology. Experimental mice study
Claudiu Saracut1, Calin Molnar1, Cristian Russu1
1Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
Acta Cirurgica Brasileira
|October 15, 2015
Summary
Deoxycholic acid (DOC) and lithocholic acid (LCA) at 0.25% did not cause cancer in mice over six months. However, they did induce precancerous adenomatous colonic injuries, suggesting a potential role in early-stage colorectal disease development.
Area of Science:
- Gastroenterology
- Oncology
- Toxicology
Background:
- Bile acids, including deoxycholic acid (DOC) and lithocholic acid (LCA), are implicated in colorectal cancer development.
- Understanding the specific carcinogenic potential of these bile acids is crucial for risk assessment.
Purpose of the Study:
- To evaluate the carcinogenic role of deoxycholic acid (DOC) and lithocholic acid (LCA) in the mouse colon over a six-month period.
- To determine if a 0.25% concentration of these bile acids induces cancerous lesions.
Main Methods:
- Female C57BL6 mice were divided into four groups: control, 0.25% DOC, 0.25% LCA, and 0.125% DOC + 0.125% LCA diets.
- Gastrointestinal tracts were examined microscopically after a six-month feeding period.
- Lesions were identified and quantified using hematoxylin-eosin staining.
Main Results:
- No gastrointestinal changes were observed in the control group.
- All treated groups showed hyperplastic Peyer's patches, flat adenomas with mild dysplasia, and chronic colitis.
- LCA-fed mice exhibited the highest incidence of flat adenomas, Peyer's patch hyperplasia, and colitis.
Conclusions:
- Deoxycholic acid (DOC) and lithocholic acid (LCA) did not produce cancerous lesions in mice within six months.
- Adenomatous colonic injuries, considered precancerous, occurred more rapidly than previously reported.
- These findings suggest a role for DOC and LCA in the early stages of colorectal pathology.
